RPMI 1640 Medium without L-Glutamine, L-Serine, Methionine, HEPES (Powder)
Lieferant: US Biological
RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
- Appearance: Pale pink, homogenous, free flowing powder
- Solubility: Magenta to red, clear, complete
- Endotoxin: ≤1 EU/ml
Directions per Liter: Dissolve 10,09 g in 900 ml of ddH₂O, stirring until completely solubilised. Do NOT heat. Add 2 g sodium bicarbonate with stirring. Adjust pH of the medium to 0,1 to 0,3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilise using a 0,22 µm membrane. Aliquot into sterile containers.
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