Sie suchten nach: Enzyme und Kits für Endpunkt-PCR

Artikel-Nr: (AGLS5500-0094)

Lieferant: AGILENT

Beschreibung: Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

VE: 1 * 1 KIT

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Lieferant: AGILENT

Beschreibung: Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Lieferant: VWR Chemicals

Beschreibung: VWR® <i>Taq</i> DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

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Artikel-Nr: (1B1409-100RXN)

Lieferant: VWR Chemicals

Beschreibung: Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

VE: 1 * 1 KIT

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Lieferant: VWR Chemicals

Beschreibung: VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from <i>Thermus aquaticus</i>, and has a molecular weight of approximately 94 kDa. VWR® <i>Taq</i> DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

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Lieferant: VWR Chemicals

Beschreibung: VWR® Red <i>Taq</i> DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Lieferant: VWR Chemicals

Beschreibung: VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Lieferant: VWR Chemicals

Beschreibung: VWR® <i>Taq</i> Plus is an optimised format of <i>Taq</i> DNA polymerase master mix and, therefore, is a good alternative to <i>Taq</i> DNA polymerase and <i>Taq</i> DNA polymerase master mix.

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Artikel-Nr: (732-3267)

Lieferant: VWR Peqlab

Beschreibung: PCR-Produkte von bis zu 12 kb und bis zu 40 kb können mit den Hochleistungs-PCR-Systemen peqGOLD 'Mid Range' und' Long Range' jeweils sowohl für genomische und episomale Templates erreicht werden. Einsetzbar anstelle von <i>Taq</i>-DNA-Polymerasen in allenn PCR-Reaktionen, wenn große Fragmente mit hohen Erträgen und größter Genauigkeit amplifiziert werden sollen, einschließlich derCharakterisierungen von Sequenzen, die in λ-Phagen oder Cosmide kloniert wurden.

VE: 1 * 1 ST

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Lieferant: Quantabio

Beschreibung: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Lieferant: AGILENT

Beschreibung: This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Lieferant: VWR Chemicals

Beschreibung: This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Lieferant: VWR Chemicals

Beschreibung: The fast extract DNA solution provides rapid and efficient extraction of PCR-ready DNA from mammalian and plant tissues. DNA is ready in eight minutes. The one-reagent protocol is divided into two simple heating steps, which can be directly followed by PCR analysis, such as screening and genotyping.

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Lieferant: VWR Chemicals

Beschreibung: Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Lieferant: VWR Chemicals

Beschreibung: Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.

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Lieferant: Quantabio

Beschreibung: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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