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Real-Time PCR (qPCR): A Comprehensive Overview A real-time polymerase chain reaction (PCR or qPCR) is a molecular biology laboratory technique based on the polymerase chain reaction (PCR). It monitors the amplification of a specific DNA molecule d...

4 Components for a Successful PCR Setup Polymerase Chain Reaction (PCR) is a laboratory technique that lets scientists amplify specific DNA sequences from a small amount of starting material. The success of PCR depends on several factors including...

Pumpen scherempfindlicher Flüssigkeiten mit Peristaltikpumpen Factors Impacting Shear Sensitivity Peristaltic pumps have become the gold standard for shear-sensitive pumping applications where damage to cells or agitation of a suspension or colloi...

Basics of Centrifugation Reprinted with permission of THERMO The purpose of this tutorial is to introduce basic concepts of centrifugation including vocabulary centrifuge and rotor types separation techniques and even gradient selection. For furth...

PEIpro Poster Reproducibility & Scalability Excellent lot-to-lot protein yield reproducibility using PEIpro®. Suspension HEK-293 cells were seeded at 1×106 cells/mL in serum-free medium and transfected with PEIpro® following the standard protocol....

Attachment and differentiation of normal and transformed cells Corning Matrigel matrix certified LDEV-free and the trusted leading basement membrane provides a physiologically relevant environment for studies of cell morphology biochemical functio...

R3971_AcroSep Various filter choices – Specialized membranes and media accomodates most applications to guarantee success. Optimized outlet tips – Minimizes sample leak- age during incubation steps and reduces the presence of hanging drops followi...

MK-SF-0002_REV01_Ultraplex_qScript_XLT1step_0918.indd Reverse Transcription For more info visit: www.quantabio.com The RT Hot Start functionality of Ultraplex improves low-copy RT-qPCR performance and enables convenient room temperature reaction s...

Application COD measurement in waste water (rapid-digestion method) Photometric determination of chromate consumption subsequent to digestion in 20 min Materials and reagents: Reagents Cat.No. 114560 Spectroquant® COD Cell Test measuring range 4.0...

solid phase techniques Thermo Scientific Solid Phase Guide H H NNHHHHH CCC H C HH 3 H 3 C SSS N H NN H N HHH 222 OO N HHH 2222 O OOOO OO H C H C H C H C 33 HHHH an introduction to H H N H CH 3 H 3 C S NH 2 NH 2 O NH 2 O H O O O O H CH 3 H Surface ...

Nalgene Rapid-Flow Filters the last line of defense against contamination Thermo Scientific Nalgene™ Rapid-Flow™ Filters All Nalgene filters now have the Rapid-Flow multi-column membrane-support system. This proprietary system provides a uniform c...

37 | VWR CLEANING OF COMPOUNDING ASEPTIC ISOLATORS (CAIS) AND CLASS II BIOLOGICAL SAFETY CABINETS (BSCS) Overview Compounding Aseptic Isolators and Class II biological safety cabinets are used in hospital pharmacies and other dispensing facilities...

User convenience in photometric applications Thermo Scientific Multiskan FC Microplate Photometer Thermo ScientificTM MultiskanTM FC – Filter-based Microplate Photometer for Research and Routine Applications The Thermo Scientific Multiskan FC micr...

Abundant Protein Depletion of Human Plasma Samples – A Reproducibility and Scaling Study Sergei I. Snovida1; Katherine E. Herting1; Ramesh Ganapathy1 Ryan Bomgarden1 Barbara J. Kaboord1 Chris Etienne1 John. C. Rogers1 ; Thermo Fisher Scientific Ro...

Velocity-Profile Deviations Influence Flowmeter Performance Selecting the right flowmeter for the liquid By Corte Swearingen Reprinted from Chemical Processing magazine Close your eyes and imagine a world in which all fluids flowing down pipes are...

Introduction to SDS-PAGE – Separating Proteins Based on Molecular Weight Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis or SDS-PAGE is a widely used technique for separating proteins based on their molecular weight. In this technique pr...