GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
The specificity of this antibody has been confirmed by WB.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:500 is recommended for IC. This antibody works well on frozen sections, cells in tissue culture and on formalin fixed histological sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Glial Fibrillary Acidic Protein
Clonality: Monoclonal
Clone: 5C10
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Rat, Mouse, Bovine, Pig