Antibody labelling kits, DyLight™
Lieferant: Thermo Fisher Scientific
The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.
- Fast – fluorescently label and purify protein in approximately one hour
- Amine-reactive dyes – label virtually any protein
- Pre-measured fluorescent dye – eliminate the time, waste and hassle associated with weighing dye
- Efficient non-reacted dye removal
- Minimal sample dilution
- Spin column format eliminates the need for column preparation, fraction screening and waiting for protein to emerge from column
- Easy protocol
The DyLight™ Microscale Antibody Labeling Kits contain all the necessary components to perform five separate labeling reactions using 100 μg of IgG. The labeling kits use high-performance spin desalting columns to provide exceptional dye removal and antibody recovery.
Invitrogen DyLight™ Antibody Labeling kits enable the creation of fluorescent antibodies that are covalently linked to different DyLight™ fluorophores. The fluorescently labeled antibodies can be used in a wide variety of laboratory applications, including immunofluorescence microscopy, IHC, western blotting, ELISA, flow cytometry, or in vitro and in vivo fluorescent detection strategies.
DyLight™ dyes exhibit higher fluorescence intensity and photostability than CyDye and LI-COR™ dyes in many applications and remain highly fluorescent over a broad pH range (pH 4–9).
Chemical Reactivity: Amine
Labeling Target: Antibodies (General), Proteins (General)
Lieferumfang: Standard kit includes Reaction Buffer (1 ml), spin columns (6 each), microcentrifuge collection tubes (12 each) and purification resin (5 ml). Each Microscale kit includes Reaction Buffer (1 ml), spin columns (5 each), microcentrifuge collection tubes (10 each) and purification resin (5 ml).
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