4',6-Diamidin-2-phenylindol-dihydrochlorid (DAPI), gelbes Pulver
Lieferant: MP Biomedicals
4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.
It binds to double-stranded DNA by forming a stable complex which fluoresce. DAPI selectively forms complexes with A-T, A-U and I-C base pairs (preferably A-T rich DNA), having one molecule of dye for every 3 base-pairs. DAPI in aqueous solution has two absorbance maxima, at 349 nm and at 263 nm. It can be used as a vital dye to stain nuclei in mature living pollen grains in conjuction with fluroescein diacetate.
Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Slightly soluble in water (10 mg/mL - clear, yellow to yellow-green solution - heat or sonication may be required).
Formel:
C₁₆H₁₅N₅·2HCl Molecular Weight: 350,25 g/mol Lagertemperatur: Gefrierschrank |
MDL:
MFCD00012681 CAS-Nummer: 28718-90-3 |
Spezifikation Testergebnisse
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