Betain Verstärker-Lösung 5 M (5X) für PCR
Lieferant: VWR Chemicals
Synonyme:
TMG
Betain-Enhancer ist besonders wirksam, wenn er mit GC-reichen Regionen oder Templates mit einem hohen Grad an Sekundärstrukturen verwendet wird. Er hat eine absenkende Wirkung auf die Primer-Schmelztemperatur.
- Enhances amplification on GC-rich and difficult DNA targets
- Lowers the melting temperature
The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.
Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₅H₁₁NO₂ Molecular Weight: 117,15 g/mol Lagertemperatur: Gefrierschrank |
MDL:
MFCD00012123 CAS-Nummer: 107-43-7 |
Spezifikation Testergebnisse
Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions | Passes test |
Volume check- fill volume >= 0.1ml per tube | Passes test |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...