Alcohol dehydrogenase (from Saccharomyces cerevisiae)
Lieferant: Merck
ADH (alcohol dehydrogenase) is a enzymes for biofuel cell research.
- Purified by crystallisation
- Solids containing ≤2% citrate buffer salts
- Soluble in water (1,0 mg/ml) - clear to slightly hazy, colourless to faintly yellow
- Extinction Coefficient: E(1%) = 14,6 (water, 280 nm)
Alcohol dehydrogenase is a tetramer containing 4 equal subunits. The active site of each subunit contains a zinc atom. Each active site also contains 2 reactive sulphydryl groups and a histidine residue. Contains bound β-NAD and β-NADH and is not suitable for the recycling microassay of β-NAD and β-NADH.
Compounds that react with free sulfhydryls, including N-alkylmaleimides and iodoacetamide. Zinc chelator inhibitors, including 1,10-phenanthroline, 8-hydroxyquinoline, 2,2′-dipyridyl, and thiourea inhibits the enzymic activity.
Synonyms: ADH, alcohol dehydrogenase from yeast, alcohol:NAD+ oxidoreductase.
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