Cis-reporting systems, PathDetect
Lieferant: STRATAGENE
The PathDetect cis-reporting systems is designed for rapid and convenient assessment of the in vivo activation of these pathways. Each cis-reporter plasmid contains the luciferase reporter gene driven by a basic promoter element (TATA box) joined to tandem repeats of various binding elements.
- Identify pathway-specific transcription activation
- Easier and faster than blotting or gel-shift assays
- Sensitive, quantitative assays
When PathDetect cis-reporting and expression plasmids encoding the gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element.
Bitte beachten For research use only. Not for use in diagnostic procedures.
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