BS3 (Bis(sulphosuccinimidyl) suberate sodium salt) ≥93% (durch NMR), Pierce™
Lieferant: Thermo Fisher Scientific
Synonyme:
BS3
Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
- Reactive groups: sulfo-NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Water-soluble; compare with DSS
- Membrane-impermeable, allowing for cell surface labeling
- High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates
BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ∼100 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker (e.g., to effect intracellular crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.
Bitte beachten For Research Use Only. Not for use in diagnostic procedures.
Formel:
C₁₆H₁₈N₂Na₂O₁₄S₂ Molecular Weight: 572,43 g/mol Lagertemperatur: Kühlschrank |
MDL:
MFCD00054982 CAS-Nummer: 82436-77-9 |
Spezifikation Testergebnisse
Description | Pierce BS3 (Sulfo-DSS) |
Product Type | Crosslinker |
Reactivity | Sulfo-NHS Ester |
Solubility | Water |
Product Line | Pierce™ |
Form | Powder |
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