The tissue genomic DNA extraction Kit is designed for the rapid purification of highly pure genomic DNA from a wide variety of samples, including whole blood, culture cells, animal tissues, mouse tails, bacteria, yeats, clinic samples (serum, plasma, feces, orine), forensic samples and paraffin-embedded tissues.
- No organic extraction or alcohol precipitation
- Complete removal of contaminants and inhibitors for reliable downstream applications
- Typical yield: 25 to 35 g genomic DNA
- Binding capacity: 60 µg
- Elution volume: 50 to 200 µl
- High quality DNA obtained that can be directly used in PCR, Southern, any enzymatic reaction, cloning, etc.
Cell lysis is achieved by incubation of the sample in a solution containing SDS and proteinase K at 55 °C. Appropriate conditions for binding DNA to the glass fiber membrane are created by addition of chaotropic ions to the lysate. Contaminants are removed by efficient washing with wash buffer. Pure genomic DNA is finally eluted with an elution buffer and it’s ready to use for subsequent reactions.
Application: For rapid purification of high-quality, ready-to-use DNA from various samples - 200 µl whole blood , 200 µl clinic samples, 200 µl buffy coat, 104 to 106 culture cells, 25 to 50 mg tissue, 0,2 to 0,5 cm mouse tail, Paraffin-embedded tissues.
