This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).
- Amplification and detection of HLVd, LCV, CanCV (simultaneously)
- Transcription ensured by the enzyme reverse transcriptase
- Real-time PCR with rapid hot-start Taq DNA polymerase
- Internal Control to exclude false-negative results
- Optimised to handle PCR inhibitors, containing UDG enzyme
- Higher stability, enzymes in seperate tubes
- PCR- platforms: runs on all established standard real-time PCR- platforms
Detection of Hop latent viroid (HLVd), Lettuce Chlorosis Virus (LCV), and Cannabis Cryptic Virus (CanCV) in Cannabis or other plant samples. This kit combines a reverse transcriptase with a multiplex qPCR assay to simultaneously detect HLVd, LCV, CanCV and an internal control. The kit is suitable for use in real time instruments with FAM, Cy5, HEX/VIC and ROX/Texas Red detection channels.
This kit uses Reverse Transcriptase (RT) real-time PCR for the detection of HLVd, LCV, CanCV in a simple, reliable, and rapid procedure. The assay combines a reverse transcriptase with a multiplex qPCR assay based on 5' nuclease real time PCR reactions to amplify a unique genomic sequence of each target. The carefully designed primers and probe ensure the highest sensitivity and specificity. The kit consists of an assay mix for detecting the targets, a positive control, and internal control (IC).To minimise PCR cross-contamination, the kit contains dUTP and uracil-N glycosylase (UDG).
Channel Target: FAM, HEX/VIC and ROX/Texas Red
Channel IC: Cy5
