Amicon® Pro Affinity Concentration Kit, Ultra-0.5 Device

Lieferant: Merck
ACK5100PG ACR5000PG ACK5003GS ACK5003NT ACK5003PA ACK5003PG ACK5010GS ACK5010PA ACK5010PG ACK5030GS ACK5030NT ACK5030PG ACK5050GS ACK5050NT ACK5050PA ACK5050PG ACK5100GS ACK5100NT ACK5100PA ACR5000PA
MILFACK5100PGEA 0 EUR
MILFACK5100PG MILFACR5000PG MILFACK5003GS MILFACK5003NT MILFACK5003PA MILFACK5003PG MILFACK5010GS MILFACK5010PA MILFACK5010PG MILFACK5030GS MILFACK5030NT MILFACK5030PG MILFACK5050GS MILFACK5050NT MILFACK5050PA MILFACK5050PG MILFACK5100GS MILFACK5100NT MILFACK5100PA MILFACR5000PA
Amicon® Pro Affinity Concentration Kit, Ultra-0.5 Device
Filter Zentrifugaleinheiten
A centrifugal tool for purifying and concentrating His-tagged proteins, GSH-tagged proteins and antibodies.

  • Minimum final concentrate volume: 15 µl
  • Colour code: Clear with pink cap
  • Non sterile
  • Ø×L: 297×120 mm

The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange. Featuring a large reservoir that accommodates a range of sample volumes, the device reduces the need for multiple centrifugation steps. Simply attach the included Amicon® Ultra filter for simultaneous elution, concentration and highly efficient buffer exchange (>99%) in a single spin.

Material:
Amicon Ultra styrene-butadiene device
Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding)
Polyethylene cap
Polyethylene frit
Polypropylene device (for exchange)
Polypropylene frit
Polypropylene tube (AU0.5; for collection)
Polypropylene tube (for collection)
Polypropylene tube (holder)

Applications: Affinity purification, depletion of abundant proteins, protein enrichment, buffer exchange, desalting, clean-up of antibody labelling reactions

Lieferumfang: Amicon® Pro affinity concentration kit Ni-NTA:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml HisBind Ni-NTA resin (Binding capacity: 5 to 10 mg His tagged protein/ml agarose), 25 ml bind buffer (4X), 25 ml wash buffer (4X), 5 ml elution buffer (4X).

Amicon® Pro affinity concentration kit Protein A:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml Protein A resin (Binding capacity: 20 mg human IgG/ml agarose), 2×66 ml bind/wash buffer, 12 ml elution buffer, 2,5 ml neutralisation buffer.

Amicon® Pro affinity concentration kit Protein G:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml Protein G resin (Binding capacity: 20 mg human IgG/ml agarose), 2×66 ml bind/wash buffer, 12 ml elution buffer, 2,5 ml neutralisation buffer.

Amicon® Pro affinity concentration kit GST:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml GST bind resin (Binding capacity: 5 to 8 mg GST tagged protein/ml agarose), 125 mg glutathione (reduced, free acid), 25 ml GST bind/wash buffer (10X), 5 ml GST reconstitution buffer (10X)
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