Sie suchten nach: Texwipe

Lieferant: Biowest

Beschreibung: L-Glutamin ist eine essentielle Aminosäure, die von praktisch allen in Kultur gezüchteten Säugetier- und Insektenzellen benötigt wird. Es ist ein entscheidender Bestandteil vieler Zellkulturmedien und dient als wichtige Energiequelle für Zellen in Kultur. L-Glutamin ist als Trockenpulver und als gefrorene Lösung sehr stabil. In flüssigen Medien oder Stammlösungen kann L-Glutamin jedoch relativ schnell abgebaut werden. L-Glutamin ist labiler in Zellkulturlösungen als andere Aminosäuren.

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Lieferant: Biowest

Beschreibung: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Artikel-Nr: (L0630-100)

Lieferant: Biowest

Beschreibung: Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

VE: 1 * 100 mL

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Artikel-Nr: (X0557-100)

Lieferant: Biowest

Beschreibung: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

VE: 1 * 100 mL

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Lieferant: Biowest

Beschreibung: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

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Lieferant: Biowest

Beschreibung: The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

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Lieferant: Biowest

Beschreibung: Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

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Lieferant: Biowest

Beschreibung: Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Zertifikate

Lieferant: Biowest

Beschreibung: Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

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Lieferant: Biowest

Beschreibung: Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

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Lieferant: Biowest

Beschreibung: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

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Artikel-Nr: (L0018-100)

Lieferant: Biowest

Beschreibung: Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.

VE: 1 * 100 mL

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Lieferant: Biowest

Beschreibung: Sterile filtered, South American origin foetal bovine serum (FBS) is derived from clotted whole blood aseptically collected from foetus via cardiac puncture. Each manufactured batch is rigorously controlled, from the collection of serum through to final packaging.

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Lieferant: Biowest

Beschreibung: Sterile filtered foetal bovine serum (FBS) derived from clotted whole blood aseptically collected from foetus via cardiac puncture. Each manufactured batch is rigorously controlled, from the collection of serum through to final packaging.

Lieferant: Biowest

Beschreibung: Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

Lieferant: Biowest

Beschreibung: Mouse serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Mouse serum is used as a blocking agent in immunoassays and controls.

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