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Beschreibung: Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of <i>Taq</i> DNA polymerase, and 6-fold lower than that of <i>Pfu</i> DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than <i>Pfu </i>DNA polymerase.

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Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

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Beschreibung: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

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Beschreibung: Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

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Beschreibung: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

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Beschreibung: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

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Beschreibung: Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.

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Beschreibung: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

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Beschreibung: Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

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Beschreibung: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

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Beschreibung: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® Hot Start II High-Fidelity DNA-Polymerase ermöglicht die Verwendung von einer Vielzahl von Primern, einschließlich derjenigen mit niedrigen Schmelztemperaturen. Phusion® Hot Start II High-Fidelity DNA-Polymerase eignet sich ideal für PCR-Anwendungen, von PCR-Routineanwendungen zu äußerst anspruchsvollen Anwendungen wie Klonen und PCR-Verfahren mit hohem Durchsatz. Sie bietet eine einzigartige dsDNA-bindende Domäne, wodurch dieser Pyrococcus-ähnliche Proofreading-DNA-Polymerase extrem robust, schnell und präzise ist.