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Beschreibung: Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.

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Beschreibung: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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Beschreibung: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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Beschreibung: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Zertifikate

Lieferant: TriLink BioTechnologies

Beschreibung: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Lieferant: TriLink BioTechnologies

Beschreibung: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

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Beschreibung: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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Beschreibung: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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Beschreibung: <p>Cas9 Nickase mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.</p>

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Beschreibung: CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

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Beschreibung: TriLink's patented CleanCap® Reagent M6 [CleanCap m6AG (3′ OMe)], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base-modified Cap 1. Cap-1 mRNAs have superior <em>in vivo</em> activity compared to Cap-0 mRNAs produced by legacy capping methods.

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Beschreibung: The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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Beschreibung: mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma sp.</em> mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

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Beschreibung: <p>Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes </em>SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.</p>

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Beschreibung: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Lieferant: TriLink BioTechnologies

Beschreibung: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.