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Gibson Assembly® HiFi 1 Step, Master mixes

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

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Gibson Assembly® Ultra kit

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

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Gibson Assembly® Ultra, Master mixes

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

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Accessories for Gibson Assembly® HiFi HC Cloning Kits

Artikel-Nr: (SYGEGA1000-05C)

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: Gibson Assembly® Positive Control, 5 reactions

VE: 1 * 5 Tests

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Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Gibson Assembly® Site directed mutagenesis kit

Artikel-Nr: (GA2100-S)

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping ends. Following mutagenesis, DNA fragments of various lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach lends itself to small and large constructs and encompasses both single and multiple insert assemblies.

VE: 1 * 5 Tests

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DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Lieferant: SGI - DNA Synthetic Genomics

Beschreibung: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

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