Human Thioredoxin 1 (Trx1) Inhibitor Screening Kit (Fluorometric)
Lieferant: Abcam
Simplest assay to screen/study/characterize thioredoxin 1 inhibitors.
- Rapid and sensitive
- Convenient and high-throughput adaptable
- Can be completed in less than one hour
- With inhibitor control
The human Thioredoxin 1 (Trx1) is a key enzyme that protects cells from oxidative stress by reducing disulfides and methionine sulfoxide groups in oxidized proteins. It also regulates DNA repair and apoptosis. In mammals, Trx1 is necessary for growth and development.
An aberrant elevated expression of Trx1 is known to promote lung, cervical, pancreatic, colorectal, and breast cancers. In this kit, Trx1 reduces quenched di-FITC-labeled oxidized glutathione (Trx1 substrate), to mono-FITC-labeled unquenched reduced glutathione. In the presence of a Trx1 inhibitor (control inhibitor included), the reaction is impeded resulting in lower or no signal.
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