Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other.
- Recommended dilution: Assay dependent
Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds.
For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.
Epitope recognized by this antibody is located at 85 kDa from N-terminus and is highly conserved (no photo-conversion).
Type:
Antigen: Phy
Clonality: Monoclonal
Clone: Pea-25
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Avena sativa, Pisum sativum