Anti-MSH6 Rabbit Polyclonal Antibody (Alexa Fluor® 555)

Lieferant: Bioss
BS-3804R-A555
BOSSBS-3804R-A555EA 893 EUR
BOSSBS-3804R-A555
Anti-MSH6 Rabbit Polyclonal Antibody (Alexa Fluor® 555)
Antikörper
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Recommended Dilutions: IF(IHC-P): 1:50-200

Type: Primary
Antigen: MSH6
Clonality: Polyclonal
Clone:
Conjugation: Alexa Fluor® 555
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat
Bestellen

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Mehr About VWR