Anti-BDNF Mouse Monoclonal Antibody [clone: 4C8]

Lieferant: Biosensis
M-1744-100
BSENM-1744-100EA 398 EUR
BSENM-1744-100
Anti-BDNF Mouse Monoclonal Antibody [clone: 4C8]
Antikörper
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.

ELISA: 50-100 ng/mL
Western Blotting (denaturing and reducing): 0.2 to 2 µg/mL. M-1744-50 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-50 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-50 will detect proBDNF in purified form on blots. Additionally, M-1744-50 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).
Immunofluorescence: 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized.
M-1744-50 is not recommended for Flow Cytometry at this time.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: BDNF
Clonality: Monoclonal
Clone: 4C8
Conjugation: Unconjugated
Epitope: Recombinant human mature BDNF expressed in E.coli
Host: Mouse
Isotype:
Reactivity: Human;Mouse;Rat;Guinea Pig
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