2',7'-Dichlorofluorescin diacetate ≥95% (durch HPLC)
Lieferant: ENZO LIFE SCIENCES
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Achtung
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Synonyme:
H2DCFDA, 2,7-Dichlorodihydrofluorescein diacetate, DCDHF diacetate
Peroxynitrite indicator.
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalysed cleavage of the diacetate groups, DCDHF is oxidised by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502 nm, Em(max): 523 nm), or by absorbance spectroscopy at 500 nm (ε = 59, 500 M⁻¹cm⁻¹). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidise DCDHF.
25 mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide, sparingly soluble in aqueous buffers.
Formel:
C₂₄H₁₆Cl₂O₇ Molecular Weight: 487,285 g/mol Lagertemperatur: Gefrierschrank |
MDL:
MFCD00128955 CAS-Nummer: 4091-99-0 |
Spezifikation Testergebnisse
Purity | ≥95% (HPLC) |
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