Fluor-de-Lys® HDAC8 deacetylase Substrate

Lieferant: ENZO LIFE SCIENCES

BMLKI1780005
ENZOBMLKI1780005EA 261 EUR
ENZOBMLKI1780005
Fluor-de-Lys® HDAC8 deacetylase Substrate
Proteine und Peptide

Fluor de Lys®-HDAC8 is a fluorogenic, diacetylated peptide substrate for HDAC8 (histone deacetylase-8).


Based on residues 379-382 of p53 (Arg-His-Lys(Ac)-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases (lysines 381, 382), it was the best for HDAC8 from among a panel of substrates patterned on p53, histone H3 and histone H4 acetylation sites.


Fluor de Lys®-HDAC8 is deacetylated by HDAC8 at a rate of more than 10-fold that of the acetylated lysine substrate, Fluor de Lys® (BML-KI104; substrates both at 100 µM).


Although named because of HDAC8’s preference for it, it is also an excellent substrate for SIRT1 (BMl-SE239; 5x the rate of BML-KI104 at 25 µM, 0.5 mM NAD+), SIRT2 and HeLa Nuclear Extract (BML-KI140; 3x KI-104 rate, 25 µM).


Must be used in conjunction with Fluor de Lys® Developer II (BML-KI176). Sufficient for 100-200 assays of human recombinant HDAC8 (BML-SE145; 1 U/well, 50-100 µM substrate).

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