Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2

Lieferant: ENZO LIFE SCIENCES

BMLP2760001
ENZOBMLP2760001EA 212 EUR
ENZOBMLP2760001
Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2
Proteine und Peptide

MMP substrate


Sensitive fluorogenic substrate for most matrix metalloproteinase (MMP) enzymes, as follows [kcat/Km (M-1s-1) and Km (µM), in parentheses]: MMP-1 (9,2×104, 27); MMP-2 (6,9×105, >30); MMP-3 (2,0×104); MMP-7 (2,9×105); MMP-8 (4,2×105); MMP-9 (6,2×105, >30); MMP-12 (1,3×105, 130); MMP-13 (1,1×106, 5,2); MMP-14 (1,3×106, 7,9); mouse, rat, and monkey MMP-14; MMP-16; MMP-20; ADAM10 (1,1×103); ADAM17/TACE (7,8×105, 26), and BACE2 (internal data using Prod. No. BML-SE550). It is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Can be used to measure cell-associated activity, or activity in cell-conditioned medium, or in biological fluids such as synovial fluid. Mca fluorescence is quenched by the Dpa group until cleavage separates them (MMPs cleave between Gly-Leu). Ex.: 328 nm, Em.: 400 nm, but it is possible to use Ex./Em. 320-340/393-420 nm. This highly-quenched (98.4%) substrate is useful for inhibitor screening and kinetic analysis.

Bestellen


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Mehr About VWR