Cyanine 3-NHS ester chloride, CELLESTIAL® fluoreszierender Farbstoff
Lieferant: ENZO LIFE SCIENCES
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Gefahr
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Synonyme:
Cy3 NHS ester, 1-[6-(2,5-Dioxopyrrolidin-1-yloxy)-6-oxohexyl]-3,3-dimethyl-2-[(1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-enyl]-3H-indolium chloride, Cyanine 3 N-hydroxysuccinimide ester, Cyanine 3 monosuccinimidyl ester, eq Cy3® NHS ester
Enzo cyanine 3 NHS-ester is a reactive, water-soluble fluorescent dye that provides bright orange signal.
- Wavelength Maxima: Excitation 553nm, Emission 570nm
The dye can be chemically linked to either nucleic acids or proteins and is suitable for a wide variety of applications including microarray analysis and protein tagging. In microarray experiments, DNA or RNA samples are labeled with the cyanine 3 and cyanine 5 dyes for differential display analysis. Since NHS esters react only with aliphatic amine groups, which nucleic acids lack, nucleotides must be modified first with aminoallyl groups thru incorporating aminoallyl-modified nucleotides during synthesis. Alternatively, labeling can be performed directly by nick translation using cyanine dUTPs (Gold 550 dUTP, Prod. No. ENZ-42521, and Red 580 dUTP, Prod. No. ENZ-42844). For protein labeling, it is critical to control the number of dye molecules affixed per protein to maintain maximal activity. For example, no more than 8 dye molecules should be bound per antibody molecule, while annexin V will only tolerate 1-2 dye molecules per protein without loss of activity.
Bestellinformation: Contents: 12 packets each containing 50nmoles of dried cyanine 3-NHS.
Formel:
C₃₄H₄₀ClN₃O₄ Molecular Weight: 590,15 g/mol Lagertemperatur: Kühlschrank |
CAS-Nummer:
146368-16-3 |
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