EQ1 NHS ester Amin-reaktiver Quencher-Farbstoff
Lieferant: ENZO LIFE SCIENCES
Amine-reactive quencher dye has a broad visible absorption but no detectable fluorescence emission in fluorescence resonance energy transfer (FRET) applications.
- Commonly used in molecular biology FRET applications to quench fluorophores
- NHS ester quencher dye can be conjugated to amine-labeled probes
- Enable multiplexing of probes with the use of different reporter-quencher dye pairs
- Compatible with most common commercially available fluorophores
EQ1 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
It can be used to quench reporter dyes: FAM, Cy2, TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Quasar® 570, CAL Fluor® Gold 540, CAL Fluor® Orange 560, CAL Fluor® Red 590, CAL Fluor® Red 610, Alexa Fluor® 430, Alexa Fluor® 488, Alexa Fluor® 532, Alexa Fluor® 546, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594, DyLight® 488, DyLight® 550, DyLight® 594, ATTO 488, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594.
Solubility: Soluble in DMSO or DMF.
Applications: Fluorescent detection, FRET
Shipping: Shipped on Dry Ice
Long Term Storage: -20°C
Use/Stability: Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
Handling: Protect from moisture. Protect from light.
Scientific Background: The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.
Spezifikation Testergebnisse
Excitation maximum | 535 nm |
Emission maximum | none |
Extinction Coefficient | 39,200 M-1cm-1 |
Correction Factor (260 nm) | 0.34 |
Correction Factor (280 nm) | 0.27 |
Purity | ≥80% (HPLC) |
Appearance | Dark red solid |
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