EQ0 NHS ester Amin-reaktiver Quencher-Farbstoff

Lieferant: ENZO LIFE SCIENCES

ENZCHM2080001
ENZOENZCHM2080001EA 130 EUR
ENZOENZCHM2080001
EQ0 NHS ester Amin-reaktiver Quencher-Farbstoff
EQ0 NHS ester

Amine-reactive quencher dye has a broad visible absorption but no detectable fluorescence emission in fluorescence resonance energy transfer (FRET) applications.


  • Commonly used in molecular biology FRET applications to quench fluorophores
  • NHS ester quencher dye can be conjugated to amine-labeled probes
  • Enable multiplexing of probes with the use of different reporter-quencher dye pairs
  • Compatible with most common commercially available fluorophores


EQ0 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.


It can be used to quench reporter dyes: AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF™ 350, CF™405S, CF™405M, Pacific Blue™, Cascade Blue®, Marina Blue®, Alexa Fluor® 350, Alexa Fluor® 405, DyLight® 350, DyLight® 405, ATTO 390, ATTO 425, ATTO 465, iFluor™350, iFLuor™405, BD Horizon V450, BUV395, eFluor®450.


Solubility: Soluble in DMSO or DMF.
Applications: Fluorescent detection, FRET
Shipping: Shipped on Dry Ice
Long Term Storage: -20°C
Use/Stability: Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
Handling: Protect from moisture. Protect from light.
Scientific Background: The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.

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Spezifikation Testergebnisse

Excitation maximum 445 nm
Emission maximum none
Extinction Coefficient 23,600 M-1cm-1
Correction Factor (260 nm) 0.48
Correction Factor (280 nm) 0.42
Purity ≥80% (HPLC)
Appearance Red brown solid

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