EQ0 NHS ester Amin-reaktiver Quencher-Farbstoff
Lieferant: ENZO LIFE SCIENCES
Amine-reactive quencher dye has a broad visible absorption but no detectable fluorescence emission in fluorescence resonance energy transfer (FRET) applications.
- Commonly used in molecular biology FRET applications to quench fluorophores
- NHS ester quencher dye can be conjugated to amine-labeled probes
- Enable multiplexing of probes with the use of different reporter-quencher dye pairs
- Compatible with most common commercially available fluorophores
EQ0 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
It can be used to quench reporter dyes: AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF™ 350, CF™405S, CF™405M, Pacific Blue™, Cascade Blue®, Marina Blue®, Alexa Fluor® 350, Alexa Fluor® 405, DyLight® 350, DyLight® 405, ATTO 390, ATTO 425, ATTO 465, iFluor™350, iFLuor™405, BD Horizon V450, BUV395, eFluor®450.
Solubility: Soluble in DMSO or DMF.
Applications: Fluorescent detection, FRET
Shipping: Shipped on Dry Ice
Long Term Storage: -20°C
Use/Stability: Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
Handling: Protect from moisture. Protect from light.
Scientific Background: The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.
Spezifikation Testergebnisse
Excitation maximum | 445 nm |
Emission maximum | none |
Extinction Coefficient | 23,600 M-1cm-1 |
Correction Factor (260 nm) | 0.48 |
Correction Factor (280 nm) | 0.42 |
Purity | ≥80% (HPLC) |
Appearance | Red brown solid |
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