Pierce™ Biotin Quantitation Kit
Lieferant: Thermo Fisher Scientific
Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
- Requires a plate reader or spectrophotometer set to measure at 500 nm
- Proven, well-characterised method for determination of biotinylation levels
- Adaptable to spectrophotometer cuvettes or standard plate readers with 96-well microplates
- Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions
- HABA-avidin complex can be used over a wide range of pH and salt concentrations
HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. This kit contains a premix of HABA and avidin and a biotinylated horseradish peroxidase (HRP) as positive control. The HABA-Avidin premix is supplied in convenient No-Weigh™ microtube packaging, which eliminates the difficulties associated with weighing small quantities of reagent.
Lieferumfang: Kit includes HABA-avidin premix, 24 tubes; and biotinylated horseradish peroxidase, 5 mg.
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