Western blotting system, Amersham ECL™

Lieferant: Cytiva

RPN2108
RPN2108EA 351 EUR
RPN2108
Western blotting system, Amersham ECL™
Elektrophorese-Reagenzien Western Blotting Reagents

Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.


  • Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
  • Optimized system provides rapid, sensitive results using simple protocols.
  • Designed for use with nitrocellulose and PVDF membranes.
  • High sensitivity: Up to 10 times more sensitive than colorimetric methods.
  • Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.


Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
Non-radioactive-no specialized facilities needed
Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide


Amersham ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.


ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.


Lieferumfang: Jedes System enthält genügend Reagenzien für die Detektion von 1000 cm² Membran: Anti-Maus HRP-Konjugat 100 µl; Anti-Kaninchen HRP-Konjugat 100 µl; ECL™ Detektionsreagenzien 1 und 2 für jeweils 62,5 ml; Blockierungsreagenz 5 g.

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