Tetramethylrhodamine ethyl ester perchlorate (TMRE) fluoreszierender Farbstoff
Lieferant: STEMCELL Technologies
Synonyme:
Tetramethylrhodamine Ethyl Ester Perchlorate, TMRE, 3,6-bis(Dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-xanthylium perchlorate
Cell-permeant, cationic fluorescent dye used to measure mitochondrial membrane potential.
- Excitation wavelength: 552 nm
- Emission wavelength: 574 nm
- Retention dependent on mitochondrial inner membrane potential
- Verified for use with flow cytometry
Detect changes in mitochondrial membrane potential with TMRE (Perchlorate) dye. TMRE uses a quick and simple staining protocol that is well suited for high-throughput screening and is compatible with flow cytometry.
TMRE is a cell-permeant, cationic compound that emits a fluorescent red-orange signal. It readily accumulates in the mitochondria with high membrane potential, whereas depolarized mitochondria fail to retain the dye.
TMRE has a sufficient retention time that is optimal for cell sorting for further purification and characterization (Barteneva et al. J Histochem Cytochem, 2014). TMRE does not inhibit the mitochondrial function, and can be used in the identification of apoptotic cells (Barteneva et al., 2014).
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Formel:
C₂₆H₂₇ClN₂O₇ Molecular Weight: 514,95 g/mol |
MDL:
MFCD00467973 CAS-Nummer: 115532-52-0 |
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