5(6)-Carboxyfluorescein diacetate NHS ester [CFSE (5(6)-CFDA SE)]
Lieferant: STEMCELL Technologies
Synonyme:
CFDA-SE, CFSE, Carboxyfluorescein diacetate succinimidyl ester, 5(6)-CFDA N-succinmidyl ester, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, 5(6)-CFDA SE
CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE.
- Cell proliferation and tracking dye
Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, which interacts with cellular amines via its succinimidyl groups to generate a highly fluorescent green dye that is impermeant to the cell membrane. CFDA-SE is frequently used in cell proliferation assays, as it is partitioned approximately equally between the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions. CFDA-SE is also used for motility assays and in vivo cell tracking experiments. CFDA-SE-labeled cells can be detected with any instrument/filter set compatible with fluorescein detection.
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Formel:
C₂₉H₁₉NO₁₁ Molecular Weight: 557,467 g/mol |
MDL:
MFCD00037466 CAS-Nummer: 150347-59-4 |
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