Phusion® green hot start II high-fidelity PCR master mix

Lieferant: Thermo Fisher Scientific
Phusion®
F-566SEA 219 EUR
F-566S F-566L
Phusion® green hot start II high-fidelity PCR master mix
Nukleinsäure-Reagenzien Enzyme und Kits für Endpunkt-PCR
Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

  • Reaction set up can be done at room temperature
  • Reduces non-specific amplification and prevents primer degradation during set up
  • Zero-time reactivation due to hot start technology
  • Speed of the polymerase allows short extension times
  • Robust reactions, minimal optimisation needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Phusion® green buffer

Lieferumfang: Kit contains 2X Phusion green hot start II high-fidelity PCR master mix (provides 1,5 mM MgCl₂ in the final 1X concentration), nuclease-free water, and DMSO.
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