Sie suchten nach: Extraktionspuffer

Lieferant: Thermo Fisher Scientific

Beschreibung: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Artikel-Nr: (786-411)

Lieferant: G-Biosciences

Beschreibung: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

VE: 1 * 250 mL

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Lieferant: Thermo Fisher Scientific

Beschreibung: This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Lieferant: OMEGA BIO-TEK

Beschreibung: TL-Puffer, Omega Bio-Tek

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Lieferant: G-Biosciences

Beschreibung: FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Lieferant: Thermo Fisher Scientific

Beschreibung: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays

Lieferant: Thermo Fisher Scientific

Beschreibung: The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Lieferant: Thermo Fisher Scientific

Beschreibung: RBC lysis buffer for mouse, Thermo Fisher Scientific

Sicherheitsdatenblätter Zertifikate

Lieferant: Thermo Fisher Scientific

Beschreibung: HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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Artikel-Nr: (J60132.AK)

Lieferant: Thermo Fisher Scientific

Beschreibung: Denaturing lysis buffer, Thermo Fisher Scientific

VE: 1 * 250 mL

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Lieferant: Thermo Fisher Scientific

Beschreibung: For cell lysis and protein extraction.

Sicherheitsdatenblätter Zertifikate

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Artikel-Nr: (ROCKMB-078-0015)

Lieferant: Rockland Immunochemicals

Beschreibung: Cell lysis buffer is used to lyse cells under non-denaturing conditions.

VE: 1 * 15 mL

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Lieferant: Thermo Fisher Scientific

Beschreibung: RBC lysis buffer for human, Thermo Fisher Scientific

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Artikel-Nr: (PIER78510)

Lieferant: Thermo Fisher Scientific

Beschreibung: Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

VE: 1 * 500 mL

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Lieferant: Merck Millipore (Novagen)

Beschreibung: Das Proteinextraktionsreagenz BugBuster® ist für den sanften Aufschluss der Zellwand von E. coli  konzipiert, um lösliche Proteine freizusetzen. Es bietet eine einfache, schnelle und kostengünstige Alternative zu mechanischen Methoden wie French Press oder Sonication für die Freisetzung von ausgedrücktem Zielprotein, in Vorbereitung für die Aufreinigung oder sonstige Anwendungen. Die Formulierung verwendet eine Reinigungsmittelmischung, die die Zellwandperforation ohne eine Denaturierung von löslichem Protein ermöglicht.