Sie suchten nach: Nukleinsäure-Reagenzien

Artikel-Nr: (K1612)

Lieferant: Thermo Fisher Scientific

Beschreibung: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

VE: 1 * 1 KIT

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Artikel-Nr: (PIER78870)

Lieferant: Thermo Fisher Scientific

Beschreibung: The Pierce™ yeast DNA extraction reagent kit uses Y-PER yeast protein extraction reagent to quickly extract and purify genomic and plasmid DNA from yeast.

VE: 1 * 1 KIT

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Lieferant: MP Biomedicals

Beschreibung: Das GNOME® DNA-Isolierungskit ist eine schnelle und effiziente Methode zur Isolierung hochmolekularer genomischer DNA aus Bakterien, Pflanzen, Hefen, Blutzellen und anderen Säugetierzellen und -geweben aller Art. Es sind keine organischen Extraktionen erforderlich, und pro Präparation können bis zu 100 μg DNA isoliert werden.

Lieferant: Thermo Fisher Scientific

Beschreibung: Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Artikel-Nr: (ICNA112100200)

Lieferant: MP Biomedicals

Beschreibung: The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.

VE: 1 * 200 Tests

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Lieferant: Thermo Fisher Scientific

Beschreibung: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

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Lieferant: OMEGA BIO-TEK

Beschreibung: Mag-Bind® SeqDTR™ wurde für die effektive und zuverlässige Entfernung von ungebundenen Terminatoren aus Sequenzierungsreaktion entwickelt. Die Sequenzierungsprodukte werden zuerst mit dem Mag-Bind® SeqDTR™ gemischt. Die DNA bindet sich dann selektiv an die Mag-Bind® SeqDTR™ Partikel. Mit zwei schnellen Waschschritten werden Spurenkontaminanten wie Nukleotide, Primern und kleine Nicht-Ziel-Amplifikationsprodukte entfernt.

Sicherheitsdatenblätter Zertifikate

Lieferant: FINNZYMES REAGENTS

Beschreibung: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

Lieferant: Thermo Fisher Scientific

Beschreibung: Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

Lieferant: FINNZYMES REAGENTS

Beschreibung: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Lieferant: Thermo Fisher Scientific

Beschreibung: The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Lieferant: AAT BIOQUEST

Beschreibung: Die Sanger-Sequenzierung, auch als Kettenabbruch-Synthese bekannt, ist eine Technik zur DNA-Sequenzierung, die auf dem selektiven Einbau kettenterminierender Didesoxynukleotide (ddNTPs) durch DNA-Polymerase basiert.

Artikel-Nr: (AATB17080)

Lieferant: AAT BIOQUEST

Beschreibung: Sanger method is one of the most reliable and earliest DNA sequencing methods.

VE: 1 * 1 µmol

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Artikel-Nr: (AATB17208)

Lieferant: AAT BIOQUEST

Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

VE: 1 * 1 µmol

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Artikel-Nr: (AATB17207)

Lieferant: AAT BIOQUEST

Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

VE: 1 * 1 µmol

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