Slide-A-Lyzer MINI Dialysis devices, 3,5K MWCO, are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >3,5 kDa in volumes from 10 µl to 2 ml. They were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices.
- Features of Slide-A-Lyzer MINI Dialysis Devices, 3,5K MWCO:
- Three convenient sizes - dialysis devices for 0,1, 0,5, and 2 ml samples fit standard 1,5-ml micro centrifuge, 15- and 50 ml conical tubes, respectively
- • Excellent sample recovery - low-binding plastic and small membrane surface area minimise sample loss
- • One-step protocol - pipette sample into the MINI device and place in tube containing the dialysis buffer; no laborious assembly, device preparation, or expensive equipment are required
- • 100% leak-tested - innovative design does not permit ‘wicking’ that can occur in home-made devices
- • Time saving - performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification, and conjugation
The 0,5 and 2 ml MINI dialysis devices fit in and are capped by inserting them into the included 15 and 50 ml conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimises the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI dialysis devices, low molecular weight contaminant removal and buffer exchange and desalting can be accomplished within four to eight hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume.
The 0,1 ml MINI dialysis devices are designed to be capped (caps included) and inserted into standard 1,5 ml micro-centrifuge tubes. Alternatively, as many as 25 MINI devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple samples inside a large beaker. Using the 0,1 ml MINI Device, 100 µl of pH 2,8 glycine buffer can be equilibrated to 1 L of pH 9,4 bicarbonate buffer in less than ten minutes. As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume; temperature and agitation rates; the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes; and the pH, ionic strength, and solvent characteristic of the buffers being used.
Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.