Experimentierkits, Aufreinigung des Restriktionsenzyms EcoRI

Lieferant: Edvotek
EDVO302EA 314 EUR
EDVO302
Experimentierkits, Aufreinigung des Restriktionsenzyms EcoRI
Lehrmittel Biologie-Lehrmittel Biochemistry Learning Activities
In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

  • For 5 lab groups
  • Complete in 3 hours

Also required: Electrophoresis tank, power supply, UV visualisation system, water bath, microcentrifuge, UV spectrophotometer and cuvettes, automatic micropipette with tips, ring stands and clamps, and 10 ml pipettes.

Lieferumfang: Im Kit enthalten: Anleitung, Ionenaustauschmatrix, Chromatographiesäulen, Zellextrakt von E.coli, Puffer für Äquilibrierung und Elution, Lambda-DNA, Lambda/EcoRI-Marker, KCl, Glycerin, Verdünnungs- und Reaktionspuffer, Gelladelösung, Agarose, Elektrophoresepuffer und Färbereagenz.
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