Sie suchten nach: Biochemistry Learning Activities

Artikel-Nr: (BE-404)

Lieferant: G-Biosciences

Beschreibung: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

VE: 1 * 1 KIT

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Artikel-Nr: (BE-401)

Lieferant: G-Biosciences

Beschreibung: The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO300)

Lieferant: EDVOTEK

Beschreibung: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

VE: 1 * 1 KIT

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Lieferant: EDVOTEK

Beschreibung: Die drei gebräuchlichsten Restriktionsenzyme sind EcoRI, BamHI und HindIII. Jedes der Enzyme katalysiert die Spaltung bei der jeweiligen definierten Basensequenz. Alle Enzyme sind lyophilisiert und enthalten 1500 Einheiten. Eine Einheit ist definiert als die Enzymmenge, die erforderlich ist, um 1,0 µg Lambda-DNA in 60 Minuten bei 37 °C in einer Reaktionsmischung von insgesamt 50 µl abzubauen.

Artikel-Nr: (EDVO225)

Lieferant: EDVOTEK

Beschreibung: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO266)

Lieferant: EDVOTEK

Beschreibung: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVOS-47)

Lieferant: EDVOTEK

Beschreibung: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO282)

Lieferant: EDVOTEK

Beschreibung: Anhand dieses einfachen und ungefährlichen Experiments erhalten die Studenten einen Einblick in die Enzymkatalyse, die Merkmale der Enzymaktion sowie die Beziehung zwischen Proteinstruktur und -funktion. Die Studenten führen einen Enzym-Assay durch und bestimmen die Rate der enzymatischen Reaktion.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO202)

Lieferant: EDVOTEK

Beschreibung: Die schnelle Isolierung von Plasmid-DNA in kleinem Maßstab ist ein Routineverfahren für das Screening und die Analyse rekombinanter DNA-Moleküle in Klonierungs- und Subklonierungsversuchen. In diesem Experiment isolieren die Studenten Plasmid-DNA ohne Zuhilfenahme von giftigen Chemikalien wie Phenol oder Chloroform.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO212)

Lieferant: EDVOTEK

Beschreibung: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO303)

Lieferant: EDVOTEK

Beschreibung: Es wurden vier experimentelle Module zu einem Experiment zusammengefasst. Dieses soll eine umfangreiche Einführung in die Biotechnologie unter besonderer Berücksichtigung des grün fluoreszierenden Proteins (GFP) vermitteln. Bakterienzellen werden dahin gehend transformiert, GFP zu exprimieren. Die transformierten Zellen werden anschließend kultiviert und das GFP mittels Säulenchromatographie aufgereinigt. Schließlich wird die Reinheit der Proteinfraktionen mit SDS-Polyacrylamid-Elektrophorese analysiert.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO235)

Lieferant: EDVOTEK

Beschreibung: Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO203)

Lieferant: EDVOTEK

Beschreibung: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO302)

Lieferant: EDVOTEK

Beschreibung: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

VE: 1 * 1 KIT

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Artikel-Nr: (BE-406)

Lieferant: G-Biosciences

Beschreibung: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

VE: 1 * 1 KIT

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Artikel-Nr: (BE-409)

Lieferant: G-Biosciences

Beschreibung: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

VE: 1 * 1 KIT

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