Sie suchten nach: Biologie-Lehrmittel

Artikel-Nr: (BE-404)

Lieferant: G-Biosciences

Beschreibung: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

VE: 1 * 1 KIT

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Artikel-Nr: (BE-401)

Lieferant: G-Biosciences

Beschreibung: The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

VE: 1 * 1 KIT

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Artikel-Nr: (BE-502)

Lieferant: G-Biosciences

Beschreibung: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO124)

Lieferant: EDVOTEK

Beschreibung: Dieses Experiment stellt ein Bioterrorismus-Szenario vor, wobei die Studenten einen simulierten genetischen Fingerabdrucktest zur Erkennung von Pocken untersuchen.

VE: 1 * 1 ST

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Artikel-Nr: (EDVO221)

Lieferant: EDVOTEK

Beschreibung: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

VE: 1 * 1 ST

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Lieferant: EDVOTEK

Beschreibung: Plasmid- und Lambda-DNA werden mit Restriktionsendonukleasen vorverdaut, die doppelsträngige DNA innerhalb oder in der Nähe definierter Basensequenzen erkennen und zerschneiden. Die Verdauungsprodukte werden mittels Agarose-Gelelektrophorese getrennt.

Artikel-Nr: (EDVO212)

Lieferant: EDVOTEK

Beschreibung: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO202)

Lieferant: EDVOTEK

Beschreibung: Die schnelle Isolierung von Plasmid-DNA in kleinem Maßstab ist ein Routineverfahren für das Screening und die Analyse rekombinanter DNA-Moleküle in Klonierungs- und Subklonierungsversuchen. In diesem Experiment isolieren die Studenten Plasmid-DNA ohne Zuhilfenahme von giftigen Chemikalien wie Phenol oder Chloroform.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO282)

Lieferant: EDVOTEK

Beschreibung: Anhand dieses einfachen und ungefährlichen Experiments erhalten die Studenten einen Einblick in die Enzymkatalyse, die Merkmale der Enzymaktion sowie die Beziehung zwischen Proteinstruktur und -funktion. Die Studenten führen einen Enzym-Assay durch und bestimmen die Rate der enzymatischen Reaktion.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO270)

Lieferant: EDVOTEK

Beschreibung: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

VE: 1 * 1 ST

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Artikel-Nr: (EDVO271)

Lieferant: EDVOTEK

Beschreibung: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

VE: 1 * 1 ST

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Artikel-Nr: (EDVOS-49)

Lieferant: EDVOTEK

Beschreibung: Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitrochondrial DNA and their biological father from chromosomal DNA.

VE: 1 * 1 ST

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Artikel-Nr: (EDVO300)

Lieferant: EDVOTEK

Beschreibung: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

VE: 1 * 1 KIT

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Artikel-Nr: (EDVO986)

Lieferant: EDVOTEK

Beschreibung: Beobachten Sie die Unterschiede zwischen den verschiedenen Säugerzelltypen sowie die Funktion dieser Zellen. Die Zellen werden auf Mikroskop-Objektträgern fixiert. Die Studenten färben die Zellen auf den Objektträgern an, um die morphologischen Besonderheiten der Zelltypen sichtbar zu machen. Diese Zellen sind besonders ungefährlich und eignen sich daher gut für Lehrzwecke.

VE: 1 * 1 ST

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Artikel-Nr: (EDVO223/AP08)

Lieferant: EDVOTEK

Beschreibung: In diesem Experiment nehmen transformierte Zellen ein Plasmid auf, welches das GFP-Gen (GFP: grün fluoreszierendes Protein) enthält. Das GFP-Gen wurde aus der Qualle <i>Aequorea victoria</i> isoliert. Die transformierten Kolonien, die das GFP-Protein enthalten, sind bei normalem Licht grün, fluoreszieren jedoch unter Langwellen-UV-Licht hell.

VE: 1 * 1 ST

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Artikel-Nr: (EDVO207)

Lieferant: EDVOTEK

Beschreibung: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

VE: 1 * 1 ST

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