Rat monoclonal antibody against mouse CD3, unconjugated.
This monoclonal antibody (clone 17A2) reacts with the mouse cluster of differentiation 3 (CD3) complex, which consists of epsilon, gamma, delta, and zeta polypeptide chains. CD3 is a member of the immunoglobulin superfamily and is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 protein chains assemble with chains of T cell receptor (TCR) and together they form the TCR-CD3 complex. Use this recombinant monoclonal antibody in applications requiring detection of CD3, such as flow cytometry (FC) and the unconjugated antibody in Immunoprecipitation (IP) and FC. TCR-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T cell development and function, and it represents one of the most complex transmembrane receptors. Blocking studies using a CD3E-targeting antibody suggests that the 17A2 clone of CD3 antibody recognizes an epitope on the epsilon chain of the CD3 complex. The 17A2 antibody clone is widely used as a phenotypic marker for mouse T cells. In addition, as the CD3E chain within the TCR-CD3 complex contains intracellular signaling domains, binding of the 17A2 antibody clone to CD3 can induce cell activation.
Bitte beachten Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').
Type:
Antigen: CD3
Clonality: Monoclonal
Clone: 17A2
Conjugation: Unconjugated
Epitope:
Host: Rat
Isotype: IgG2b kappa
Reactivity: Mouse