Sie suchten nach: Mercaptoethanol

Lieferant: Merck Millipore (Novagen)

Beschreibung: Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Lieferant: MP Biomedicals

Beschreibung: Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins.

Lieferant: HyClone products (Cytiva)

Beschreibung: Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.

Lieferant: MP Biomedicals

Beschreibung: Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.

Lieferant: Thermo Fisher Scientific

Beschreibung: Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

Lieferant: Biosensis

Beschreibung: X63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis. Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG1-saporin conjugates such as MC192-saporin.

Lieferant: Biosensis

Beschreibung: MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75NTR, the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis.

Artikel-Nr: (ENZOENZ42730)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.

VE: 1 * 1 KIT

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Artikel-Nr: (80-6483-56)

Lieferant: Cytiva

Beschreibung: 2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

VE: 1 * 1 KIT

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Lieferant: HyClone products (Cytiva)

Beschreibung: HyClone™ ActiPro™ cell culture media has been formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines. The cultured medium is chemically defined, animal-derived component-free (ADCF), and optimized for high-yield protein expression and production in batch or CHO fed-batch cell culture processes.

Lieferant: AGILENT

Beschreibung: High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

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Artikel-Nr: (BSENM-1719-100)

Lieferant: Biosensis

Beschreibung: TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).

VE: 1 * 100 µG

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Artikel-Nr: (PIERA35349)

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

VE: 1 * 10 mg

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Artikel-Nr: (BSENM-1714-100)

Lieferant: Biosensis

Beschreibung: The capsaicin receptor (VR1, TRPV1) is a ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. The receptor seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. It is involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH less than 6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (Ref: uniprot.org).

VE: 1 * 100 µG

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