Sie suchten nach: Thermo Fisher Scientific

Artikel-Nr: (K1441)

Lieferant: Thermo Fisher Scientific

Beschreibung: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

VE: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Artikel-Nr: (SM1821)

Lieferant: Thermo Fisher Scientific

Beschreibung: RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

VE: 1 * 1 KIT

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Artikel-Nr: (R0861)

Lieferant: Thermo Fisher Scientific

Beschreibung: Dithiothreitol (DTT, Cleland's Reagenz)

VE: 1 * 5 g

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Artikel-Nr: (SM1103)

Lieferant: Thermo Fisher Scientific

Beschreibung: FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

VE: 1 * 1 KIT

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Artikel-Nr: (SM0383)

Lieferant: Thermo Fisher Scientific

Beschreibung: MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

VE: 1 * 1 KIT

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Artikel-Nr: (ER1751)

Lieferant: Thermo Fisher Scientific

Beschreibung: CCNGGA sites and cuts best at 37 °C in Tango buffer.

VE: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

Artikel-Nr: (EN0525)

Lieferant: Thermo Fisher Scientific

Beschreibung: DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyses phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'- phosphate and 3'- OH groups.

VE: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Lieferant: Thermo Fisher Scientific

Beschreibung: Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Lieferant: Thermo Fisher Scientific

Beschreibung: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Artikel-Nr: (EN0771)

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

VE: 1 * 1 ST

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Artikel-Nr: (K0512)

Lieferant: Thermo Fisher Scientific

Beschreibung: Das DNA-Reinigungskit ist ein einfaches und schnelles System für die hochwertige Reinigung genomischer DNA aus verschiedenen Quellen, darunter: Vollblut, Serum, Zelllinien, Bakterienzellen, Pflanzen- und Säugetiergewebe.

VE: 1 * 1 KIT

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