Erythropoietin/EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesised by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
WB: 0,5 - 1 µg/ml IF: 1-2 µg/ml Immunohistochemistry (FFPE): 4-8 µg/ml for 30 min at RT (1) Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Titering of the EPO antibody may be required for optimal performance.
1. Required HIER: boil tissue sections in 10 mM citrate buffer, pH 6, for 10 - 20 min.
Type: Primary
Antigen: EPO
Clonality: Monoclonal
Clone: EPO/1368
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG
Reactivity: Human