Tyrosine-protein kinase ZAP70 is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, it phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. [UniProt].
Immunofluorescence: 1 - 2 µg/ml Immunohistochemistry (FFPE): 1 - 2 µg/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the ZAP-70 antibody may be required for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: ZAP70
Clonality: Monoclonal
Clone: ZTP70-1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human
