Sie suchten nach: Thermo Fisher Scientific

Artikel-Nr: (R0862)

Lieferant: Thermo Fisher Scientific

Beschreibung: Dithiothreitol (DTT, Cleland's Reagenz)

VE: 1 * 1 KIT

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Artikel-Nr: (ER1921)

Lieferant: Thermo Fisher Scientific

Beschreibung: CCWGG sites and cuts best at 37 °C in O buffer.

VE: 1 * 1 KIT

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Artikel-Nr: (EN0771)

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

VE: 1 * 1 ST

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Lieferant: Thermo Fisher Scientific

Beschreibung: Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Lieferant: Thermo Fisher Scientific

Beschreibung: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Zertifikate

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Artikel-Nr: (SO142)

Lieferant: Thermo Fisher Scientific

Beschreibung: Random hexamer primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Random hexamer primer has been functionally tested in first strand cDNA synthesis.

VE: 1 * 1 KIT

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Artikel-Nr: (K0512)

Lieferant: Thermo Fisher Scientific

Beschreibung: Das DNA-Reinigungskit ist ein einfaches und schnelles System für die hochwertige Reinigung genomischer DNA aus verschiedenen Quellen, darunter: Vollblut, Serum, Zelllinien, Bakterienzellen, Pflanzen- und Säugetiergewebe.

VE: 1 * 1 KIT

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Artikel-Nr: (K1612)

Lieferant: Thermo Fisher Scientific

Beschreibung: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

VE: 1 * 1 KIT

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Artikel-Nr: (ER0593)

Lieferant: Thermo Fisher Scientific

Beschreibung: GGCCGC sites and cuts best at 37 °C in O buffer.

VE: 1 * 1 KIT

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Artikel-Nr: (EP0111)

Lieferant: Thermo Fisher Scientific

Beschreibung: T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

VE: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

Lieferant: Thermo Fisher Scientific

Beschreibung: GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, Southern blotting, and enzymatic reactions.

Artikel-Nr: (R0532)

Lieferant: Thermo Fisher Scientific

Beschreibung: TurboFect™ transfection reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect™ is a sterile solution of a proprietary cationic polymer in water.

VE: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.