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Sie suchten nach: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER34042

Substrate for western blotting, 1-Step™ NBT/BCIP

Artikel-Nr: (PIER34042)
Lieferant: Thermo Fisher Scientific
Beschreibung: The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.
VE: 1 * 250 mL
Product Image-PIER34070

Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Artikel-Nr: (PIER34070)
Lieferant: Thermo Fisher Scientific
Beschreibung: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.
VE: 1 * 100 mL
Product Image-PIER34000

CN/DAB substrate kit, Pierce™

Artikel-Nr: (PIER34000)
Lieferant: Thermo Fisher Scientific
Beschreibung: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
VE: 1 * 1 KIT
Image Unavailable-786-423

High molecular weight transfer buffer (5X)

Artikel-Nr: (786-423)
Lieferant: G-Biosciences
Beschreibung: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
VE: 1 * 1 L
Image Unavailable-PIER15162

NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Artikel-Nr: (PIER15162)
Lieferant: Thermo Fisher Scientific
Beschreibung: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
VE: 1 * 1 KIT
Product Image-PIER37574

1-Step™ Ultra TMB-Blotting solution, Pierce™

Artikel-Nr: (PIER37574)
Lieferant: Thermo Fisher Scientific
Beschreibung: The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.
VE: 1 * 250 mL
Product Image-PIER20148X

Chemiluminescent EMSA kit, LightShift™

Lieferant: Thermo Fisher Scientific
Beschreibung: LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Product Image-PIER34075

Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Lieferant: Thermo Fisher Scientific
Beschreibung: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

Product Image-PIER34012

1-Step™, Pierce™ Chloronaphthol

Artikel-Nr: (PIER34012)
Lieferant: Thermo Fisher Scientific
Beschreibung: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.
VE: 1 * 250 mL
Product Image-AATB26100

Amplite® West ECL HRP Substrate *Femto Sensitivity*

Lieferant: AAT BIOQUEST
Beschreibung: Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

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Product Image-PIER34002

DAB substrate

Artikel-Nr: (PIER34002)
Lieferant: Thermo Fisher Scientific
Beschreibung: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
VE: 1 * 1 KIT
Product Image-PIER17075

Biotin random prime DNA labelling kit, North2South™

Artikel-Nr: (PIER17075)
Lieferant: Thermo Fisher Scientific
Beschreibung: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
VE: 1 * 1 KIT
Product Image-PIER31490

Horseradish peroxidase, Pierce™

Artikel-Nr: (PIER31490)
Lieferant: Thermo Fisher Scientific
Beschreibung: Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
VE: 1 * 10 mg
Product Image-PIER15165

HRP conjugates, HisProbe™

Artikel-Nr: (PIER15165)
Lieferant: Thermo Fisher Scientific
Beschreibung: HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
VE: 1 * 2 mg
Product Image-PIER21230

Pierce™ Clean-Blot™ IP Detection Kit and Reagents

Lieferant: Thermo Fisher Scientific
Beschreibung: Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

Product Image-PIER46641

Western blot enhancer, SuperSignal™, Pierce

Lieferant: Thermo Fisher Scientific
Beschreibung: SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

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