Sie suchten nach: Western Blotting Reagents

Lieferant: Rockland Immunochemicals

Beschreibung: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Artikel-Nr: (A3417.5000)

Lieferant: PanReac AppliChem

Beschreibung: Das CheLuminate-HRP PicoDetect ist ein komplettes Kit mit gebrauchsfertigen Reagenzien für die Chemilumineszenz-Nachweis von immobilisierten Proteinen (Western Blot) oder immobilisierten Nukleinsäuren (Southern oder Northern Blot), die direkt oder indirekt mit Meerrettichperoxidase (HRP) konjugiert sind.

VE: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Artikel-Nr: (RPN3006)

Lieferant: Cytiva

Beschreibung: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

VE: 1 * 1 ST

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Lieferant: Cytiva

Beschreibung: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Lieferant: Cytiva

Beschreibung: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Artikel-Nr: (RPN3005)

Lieferant: Cytiva

Beschreibung: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

VE: 1 * 1 ST

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Artikel-Nr: (RPN5785)

Lieferant: Cytiva

Beschreibung: ECF™ substrate for Western blotting

VE: 1 * 1 ST

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Lieferant: Cytiva

Beschreibung: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Sicherheitsdatenblätter Zertifikate

Lieferant: Cytiva

Beschreibung: Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Lieferant: Cytiva

Beschreibung: Chemilumineszenz-Nachweisreagenz zum Nachweis von Proteinen mit hohem bis mittlerem Vorkommen.

Artikel-Nr: (RPN2235)

Lieferant: Cytiva

Beschreibung: Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

VE: 1 * 1 KIT

Sicherheitsdatenblätter Zertifikate Sale!

Lieferant: Cytiva

Beschreibung: CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Lieferant: Cytiva

Beschreibung: Amersham™ ECL™ Prime ist ein hochsensitives Western Blotting Detektionsreagenz, das sich durch eine stabile Signalemission auszeichnet. Dies ermöglicht mehrere Belichtungen und macht es einfacher, mehrere Blots in einem Experiment parallel zu verarbeiten. Amersham™ ECL™ Prime bietet ein Detektionssystem, das über einen breiten dynamischen Bereich von Proteinmengen hinweg empfindlich, stabil und präzise quantitativ ist und sich gleichzeitig durch den niedrigen Verbrauch der teuren Antikörperreagenzien auszeichnet. Die hohe Leistung des Amersham™ ECL™ Prime ist einem Enhancer im Reagenz zu verdanken, der den Enzymumsatz steigert und somit die Signalintensität und -dauer deutlich erhöht. Das Lichtsignal wird durch Zugabe eines Katalysators weiter intensiviert.

Lieferant: Cytiva

Beschreibung: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Sicherheitsdatenblätter Zertifikate

Artikel-Nr: (RPN2125)

Lieferant: Cytiva

Beschreibung: Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

VE: 1 * 40 g

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