Artikel-Nr: (PIER77610)

Lieferant: Thermo Fisher Scientific

Beschreibung: Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.

VE: 1 * 1 SET

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Artikel-Nr: (PIER77130)

Lieferant: Thermo Fisher Scientific

Beschreibung: Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.

VE: 1 * 100 mg

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Artikel-Nr: (PIER90003)

Lieferant: Thermo Fisher Scientific

Beschreibung: Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

VE: 1 * 10 ST

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Artikel-Nr: (786-232)

Lieferant: G-Biosciences

Beschreibung: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

VE: 1 * 1 KIT

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Artikel-Nr: (786-792)

Lieferant: G-Biosciences

Beschreibung: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

VE: 1 * 1 ST

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Lieferant: Thermo Fisher Scientific

Beschreibung: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Lieferant: Thermo Fisher Scientific

Beschreibung: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Zertifikate

Artikel-Nr: (PIER89874)

Lieferant: Thermo Fisher Scientific

Beschreibung: The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

VE: 1 * 1 KIT

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Artikel-Nr: (PIER89839)

Lieferant: Thermo Fisher Scientific

Beschreibung: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.

VE: 1 * 1 KIT

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Lieferant: Merck Millipore (Novagen)

Beschreibung: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Artikel-Nr: (324715-5)

Lieferant: Merck Millipore (Calbiochem‎)

Beschreibung: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.

VE: 1 * 5 µG

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Artikel-Nr: (786-316)

Lieferant: G-Biosciences

Beschreibung: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.

VE: 1 * 1 KIT

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Artikel-Nr: (786-125)

Lieferant: G-Biosciences

Beschreibung: The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.

VE: 1 * 50 mL

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Artikel-Nr: (324708-2)

Lieferant: Merck Millipore (Calbiochem‎)

Beschreibung: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

VE: 1 * 2 µG

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Lieferant: Merck Millipore (Calbiochem‎)

Beschreibung: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.