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Anti-Nuclear Membrane Mouse Monoclonal Antibody (Biotin) [clone: NM97]

Lieferant: Biotium

Beschreibung: This antibody recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. ,The nuclear envelope (also known as the perinuclear envelope, nuclear membrane, nucleolemma or karyotheca) is the double membrane of the nucleus that encloses genetic material in eukaryotic cells. It separates the contents of the nucleus (DNA in particular) from the cytosol (cytoplasm). Numerous nuclear pores are present on the nuclear envelope to facilitate and regulate the exchange of materials (for example, proteins and RNA) between the nucleus and the cytoplasm. The space between the two membranes that make up the nuclear envelope is called the perinuclear space (also called the perinuclear cisterna), and is usually about 20 - 40 nm wide. Each of the two membranes is composed of a lipid bilayer. The outer membrane is continuous with the rough endoplasmic reticulum. The inner membrane is erected upon the nuclear lamina, a network of intermediate filaments made of lamin, that plays a role in mitosis and meiosis. The type of lamins present are A, B1, B2, and C. The nuclear envelope may also play a role in the disposition of chromatin inside the nucleus. The lamina acts as a site of attachment for chromosomes. It also acts like a shield for the nucleus. During prophase in mitosis, the chromatids begin condensing to form chromosomes, and the nuclear envelope begins to disintegrate. During metaphase, the nuclear envelope is completely disintegrated, and the chromosomes can be pulled apart as chromatids by the spindle fibers.

Streptomyces avidini Streptavidin, MP Biomedicals

Artikel-Nr: (ICNA08623001)

Lieferant: MP Biomedicals

Beschreibung: Streptavidin effectively binds to biotin with an isoelectric point close to neutral and it contains no carbohydrate residues.

VE: 1 * 1 mg

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N-Biotinyl-N'-(3-maleimidopropionyl)-3,6-dioxaoctane-1,8-diamine ≥95%

Artikel-Nr: (AATB3015)

Lieferant: AAT BIOQUEST

Beschreibung: This thiol-reactive biotin derivative contains a long arm (~20 angstrom) to increase its avidin-binding affinity.

VE: 1 * 5 mg

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Anti-Nuclear Membrane Mouse Monoclonal Antibody (Biotin) [clone: AE-5]

Lieferant: Biotium

Beschreibung: This antibody recognizes an antigen associated with the nuclear membrane in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.,The nuclear envelope (also known as the perinuclear envelope, nuclear membrane, nucleolemma or karyotheca) is the double membrane of the nucleus that encloses genetic material in eukaryotic cells. It separates the contents of the nucleus (DNA in particular) from the cytosol (cytoplasm). Numerous nuclear pores are present on the nuclear envelope to facilitate and regulate the exchange of materials (for example, proteins and RNA) between the nucleus and the cytoplasm. The space between the two membranes that make up the nuclear envelope is called the perinuclear space (also called the perinuclear cisterna), and is usually about 20 - 40 nm wide. Each of the two membranes is composed of a lipid bilayer. The outer membrane is continuous with the rough endoplasmic reticulum. The inner membrane is erected upon the nuclear lamina, a network of intermediate filaments made of lamin, that plays a role in mitosis and meiosis. The type of lamins present are A, B1, B2, and C. The nuclear envelope may also play a role in the disposition of chromatin inside the nucleus. The lamina acts as a site of attachment for chromosomes. It also acts like a shield for the nucleus. During prophase in mitosis, the chromatids begin condensing to form chromosomes, and the nuclear envelope begins to disintegrate. During metaphase, the nuclear envelope is completely disintegrated, and the chromosomes can be pulled apart as chromatids by the spindle fibers.

Affinity purification Streptavidin Resin and buffers

Lieferant: G-Biosciences

Beschreibung: Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Anti-Nucleocapsid Phosphoprotein Rabbit Polyclonal Antibody (Biotinylated)

Artikel-Nr: (PRSI9103-BIOTIN)

Lieferant: ProSci Inc.

Beschreibung: SARS-CoV-2 (COVID-19) Nucleocapsid antibody (biotin) can be stored at 4 °C for three months and −20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

VE: 1 * 100 µG

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Anti-Nucleocapsid Phosphoprotein Rabbit Polyclonal Antibody (Biotinylated)

Artikel-Nr: (PRSI9099-BIOTIN)

Lieferant: ProSci Inc.

Beschreibung: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (biotin) can be stored at 4 °C for three months and −20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

VE: 1 * 100 µG

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Streptavidin agarose, Pierce™

Lieferant: Thermo Fisher Scientific

Beschreibung: Pierce™ Streptavidin agarose is a standard-capacity crosslinked 6% beaded agarose resin of immobilised recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.

Anti-Cd3e Hamster Monoclonal Antibody [clone: 145-2C11] (Biotin)

Artikel-Nr: (PRSI76-263)

Lieferant: ProSci Inc.

Beschreibung: The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex. Together with the gamma and delta subunits of CD3, the epsilon subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the epsilon chain enhances the antigen recognition.The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes. The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.

VE: 1 * 0,1 mg

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Anti-Cd3e Hamster Monoclonal Antibody [clone: 145-2C11] (Biotin)

Artikel-Nr: (PRSI76-264)

Lieferant: ProSci Inc.

VE: 1 * 0,025 mg

Neuer Artikel bei VWR! Sale!

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D(+)-Biotin ≥99%, Reagenzqualität

Lieferant: VWR Chemicals

Beschreibung: A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection.

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