Sie suchten nach: Merck Millipore (Novagen)

Artikel-Nr: (69755-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

VE: 1 * 1 KIT

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Artikel-Nr: (69750-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

VE: 1 * 10 µG

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Artikel-Nr: (69704-4)

Lieferant: Merck Millipore (Novagen)

Beschreibung: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

VE: 1 * 10 mL

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Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Lieferant: Merck Millipore (Novagen)

Beschreibung: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Artikel-Nr: (71281-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

VE: 1 * 1 ST

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Lieferant: Merck Millipore (Novagen)

Beschreibung: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Artikel-Nr: (70856-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: Convenient solution for selective degradation of RNA.

VE: 1 * 1 mL

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Artikel-Nr: (70527-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

VE: 1 * 15 mL

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Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel-Nr: (69022-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

VE: 1 * 1 KIT

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Lieferant: Merck Millipore (Novagen)

Beschreibung: NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and <i>recA</i> and <i>endA</i> mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Das Proteinextraktionsreagenz BugBuster® ist für den sanften Aufschluss der Zellwand von E. coli  konzipiert, um lösliche Proteine freizusetzen. Es bietet eine einfache, schnelle und kostengünstige Alternative zu mechanischen Methoden wie French Press oder Sonication für die Freisetzung von ausgedrücktem Zielprotein, in Vorbereitung für die Aufreinigung oder sonstige Anwendungen. Die Formulierung verwendet eine Reinigungsmittelmischung, die die Zellwandperforation ohne eine Denaturierung von löslichem Protein ermöglicht.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Pellet Paint® Co-Precipitant ist ein sichtbarer, mit Farbstoff markierter Träger, der speziell für die Verwendung bei der Alkoholfällung von Nukleinsäuren formuliert wurde. Die 2-minütige Fällung erfordert keine Inkubation bei niedriger Temperatur oder eine längere Zentrifugation. Sowohl RNS als auch DNS werden sogar aus stark verdünnten Lösungen (2 ng/ml) effizient gefällt, und die Pellets können durch ihre klare violette Farbe leicht gefunden werden. Die Pellets können während der Waschschritte leicht verfolgt werden, wodurch Verluste während der Handhabung vermieden und gründliche Pellet-Resuspensionen gewährleistet werden.

Artikel-Nr: (72181-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: 293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

VE: 1 * 1 mL

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