Sie suchten nach: Protein-Extraktion

Artikel-Nr: (ENZOENZ450150100)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.

VE: 1 * 1 KIT

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Artikel-Nr: (ENZOENZ450130002)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.

VE: 1 * 1 KIT

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Artikel-Nr: (786-260)

Lieferant: G-Biosciences

Beschreibung: FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.

VE: 1 * 1 KIT

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Artikel-Nr: (786-241)

Lieferant: G-Biosciences

Beschreibung: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

VE: 1 * 1 KIT

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Artikel-Nr: (786-250)

Lieferant: G-Biosciences

Beschreibung: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.

VE: 1 * 1 KIT

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Lieferant: G-Biosciences

Beschreibung: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Lieferant: Biotium

Beschreibung: AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).

Lieferant: Thermo Fisher Scientific

Beschreibung: Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

Artikel-Nr: (786-360)

Lieferant: G-Biosciences

Beschreibung: Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.

VE: 1 * 1 KIT

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Artikel-Nr: (786-259)

Lieferant: G-Biosciences

Beschreibung: Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.

VE: 1 * 1 KIT

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Artikel-Nr: (ENZOBMLKI1761250)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).

VE: 1 * 1,25 mL

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Lieferant: Thermo Fisher Scientific

Beschreibung: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Lieferant: ENZO LIFE SCIENCES

Beschreibung: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

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Artikel-Nr: (786-681)

Lieferant: G-Biosciences

Beschreibung: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

VE: 1 * 1 KIT

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Artikel-Nr: (786-793)

Lieferant: G-Biosciences

Beschreibung: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

VE: 1 * 5 mL

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Lieferant: G-Biosciences

Beschreibung: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.